Rat Angiopoietin-like Protein 2

Mfr.No.

E1504Ra

Sensitivity

5.29ng/L

Detection Range

10-3500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Angiopoietin-like Protein 2 (also known as ANGPTL2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AMOTL 2;AMOTL2;Angiomotin-like protein 2

Molecular Weight

85587

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1504Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	238.6	11.64	4.9
Intra-Assay	2	18	483.1	27.37	5.7
Intra-Assay	3	18	1365.8	48.9	3.6

Background

Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC (By similarity).

Protocol

These standard curves of E1504Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.225	2.197	2.14
	2.169
1000ng/L	1.341	1.346	1.289
	1.35
500ng/L	0.887	0.834	0.778
	0.782
250ng/L	0.467	0.436	0.379
	0.405
125ng/L	0.231	0.236	0.179
	0.24
0ng/L	0.052	0.057	0
	0.061

Gene ID	65157
UniProt ID	G3V735

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112735	E1504Ra	48T	$320		Add to Cart
BHE12112735	E1504Ra	96T	$458		Add to Cart

Rat Angiopoietin-like Protein 2 - ANGPTL2 ELISA Kit

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