Rat Angiopoietin 1 - ANG-1 ELISA Kit

Mfr.No.

E0667Ra

Sensitivity

1.62ng/mL

Detection Range

3-900ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Angiopoietin 1 (also known as ANG-1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ANG 1;ANG-1;Angiopoietin-1

Molecular Weight

57461

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0667Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	72.4	3	4.1
Intra-Assay	2	18	243.7	8.33	3.4
Intra-Assay	3	18	123.3	5.29	4.3

Background

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

Protocol

These standard curves of E0667Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/mL	2.304	2.278	2.258
	2.252
240ng/mL	1.46	1.351	1.331
	1.242
120ng/mL	0.694	0.701	0.681
	0.709
60ng/mL	0.297	0.323	0.303
	0.35
30ng/mL	0.177	0.185	0.164
	0.193
0ng/mL	0.019	0.02	0
	0.021

Gene ID	89807
UniProt ID	O35460

Research Area	Cardiovascular; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111934	E0667Ra	48T	$320		Add to Cart
BHE12111934	E0667Ra	96T	$458		Add to Cart

Rat Angiopoietin 1 - ANG-1 ELISA Kit

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