Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ANA and the recovery rates were calculated by comparing the measured value to the expected amount of ANA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
94
EDTA plasma(n=5)
86-100
93
heparin plasma(n=5)
90-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-95%
92-103%
88-102%
EDTA plasma(n=5)
88-98%
88-98%
87-95%
heparin plasma(n=5)
80-92%
90-100%
85-99%
Sensitivity
< 0.281ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ANAELISA Kit allows for the in vitro quantitative determination of ANA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ANA (Anti-nuclear Antibody) ELISA Kit