Rat amyloid beta peptide 1-42,Abeta1-42 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10786r

Sensitivity

0.225 pg/mL

Detection Range

0.9 pg/mL-60 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amyloid beta peptide 1-42,Abeta1-42, Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Abeta1-42,amyloid betapeptide 1-42

Recovery

The recovery of human ANTXR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-106
EDTA plasma (n=4)	91	87-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANTXR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	90
	Range %	84-100
1:4	Average %	92
	Range %	85-102
1:8	Average %	92
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Abeta1-42

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats Ghareeb DA. et al,Evid Based Complement Alternat Med,2015 (2015 ),13 pages Modulation of LOX and COX pathways via inhibition of amyloidogenesis contributes to mitoprotection against B-amyloid oligomer-induced toxicity in an animal model of Alzheimer's disease in rats. Kalra J.et al,Pharmacol Biochem Behav.,146-147:1-12 Developmental perfluorooctane sulfonate exposure results in tau hyperphosphorylation and B-amyloid aggregation in adults rats: Incidence for link to Alzheimer's disease Zhang Q.et al,Toxicology.,2016 347-349:40-6. Neuroprotective effect of berberine against environmental heavy metals-induced neurotixicity and Alzheimer's-like disease in rats Hend M.Hussien.et al,Food and Chemical Toxicology,Nov 21 Possible role of endothelin receptor against hyperhomocysteinemia and B-amyloid induced AD type of vascular dementia in rats Singh M.et al,Brain Res Bull.,Mar 6 Catalpol provides a protective effect on fibrillary Aβ1-42 -induced barrier disruption in an in vitro model of the blood–brain barrier Liu C.et al,Phytother Res,Feb 26 Treadmill Exercise Ameliorates Spatial Learning and Memory Deficits Through Improving the Clearance of Peripheral and Central Amyloid-Beta Levels Davar Khodadadi.et al,Neurochemical Research,June 11

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