Rat amyloid beta peptide 1-40,Abeta1-40 ELISA Kit

Mfr.No.

CSB-E08302r

Sensitivity

0.156 pg/mL

Detection Range

0.625 pg/mL-40 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amyloid beta peptide 1-40,Abeta1-40, amyloid beta peptide 1-40,Abeta1-40,amyloid beta peptide 1-40

Recovery

The recovery of human ANTXR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-95
EDTA plasma (n=4)	102	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANTXR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-107
1:2	Average %	100
	Range %	91-105
1:4	Average %	94
	Range %	88-99
1:8	Average %	102
	Range %	95-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Abeta1-40

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Apurinic/apyrimidinic endonuclease 1 is a key modulator of aluminum-induced neuroinflammation Zaky A et al,BMC Neurosci,2013 Mar 11;14:26. Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats Ghareeb DA. et al,Evid Based Complement Alternat Med,2015 (2015 ),13 pages Neuroprotective effect of berberine against environmental heavy metals-induced neurotixicity and Alzheimer's-like disease in rats Hend M.Hussien.et al,Food and Chemical Toxicology,Nov 21

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