Rat Amyloid beta A4 protein(APP) ELISA kit

Rat Amyloid beta A4 protein(APP) ELISA kit

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Mfr.No.

CSB-EL001950RA

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amyloid beta (A4) precursor protein, AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, OTTHUMP00000096096, amyloid beta A4 protein, beta-amyloid peptide, cerebral vascular amyloid peptide, peptidase nexin-II, protease nexin-II

Recovery

The recovery of rat TSP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TSP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	94-100
1:200	Average %	89
	Range %	84-92
1:400	Average %	104
	Range %	100-108
1:800	Average %	96
	Range %	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.405	2.487	2.446	2.272
200	1.992	1.972	1.982	1.808
100	1.583	1.598	1.591	1.417
50	1.131	1.168	1.150	0.976
25	0.748	0.728	0.738	0.564
12.5	0.498	0.462	0.480	0.306
6.25	0.335	0.314	0.325	0.151
0	0.173	0.175	0.174

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08592

Applications	ELISA
Research Area	APP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507576	CSB-EL001950RA	96T	$595	Add to Cart
BHE10507576	CSB-EL001950RA	5x96T	$2856	Add to Cart
BHE10507576	CSB-EL001950RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.