Rat AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

Mfr.No.

ER0730

Alternative Names

AMPK ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

3.125-200ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of AMPK and the recovery rates were calculated by comparing the measured value to the expected amount of AMPK in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-103	92
EDTA plasma(n=5)	86-98	92
heparin plasma(n=5)	86-105	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-102%	94-101%	87-95%
EDTA plasma(n=5)	85-95%	83-98%	89-100%
heparin plasma(n=5)	80-91%	91-99%	86-100%

Sensitivity

< 1.875ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

AMPK

Notes

For Research Use Only

UniProt ID	Q09137
Antigen	AMPK

Applications	ELISA
Applications Notes	AMPK ELISA Kit allows for the in vitro quantitative determination of AMPK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800655	ER0730	96T	$400		Add to Cart

Rat AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

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