Rat Alpha-fetoprotein - AFP ELISA Kit

Mfr.No.

E0332Ra

Sensitivity

0.004ng/ml

Detection Range

0.01-3ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Alpha-fetoprotein (also known as AFP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AFP;AFPD;Alpha-1-fetoprotein;Alpha-fetoglobulin;Alpha-fetoprotein;FETA

Molecular Weight

68386

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0332Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	0.39	0.02	5.63
Intra-Assay	2	18	1.13	0.03	2.4
Intra-Assay	3	18	0.9	0.05	5.41

Background

Binds estrogens, fatty acids and metals.

Protocol

These standard curves of E0332Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1.6ng/ml	1.876	1.953	1.895
	2.03
0.8ng/ml	0.97	0.935	0.877
	0.899
0.4ng/ml	0.608	0.587	0.529
	0.566
0.2ng/ml	0.245	0.25	0.192
	0.256
0.1ng/ml	0.161	0.118	0.06
	0.075
0ng/ml	0.055	0.058	0
	0.061

Gene ID	24177
UniProt ID	P02773

Research Area	Cardiovascular; Signal Transduction; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111631	E0332Ra	48T	$320		Add to Cart
BHE12111631	E0332Ra	96T	$458		Add to Cart

Rat Alpha-fetoprotein - AFP ELISA Kit

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