Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

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Mfr.No.

CSB-EL001654RA

Sensitivity

0.649 ng/mL

Detection Range

1.563 ng/mL-100 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

alpha-1-microglobulin/bikunin precursor, A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, alpha-1-microglobulin/bikunin, bikunin, complex-forming glycoprotein heterogeneous in charge, growth-inhibiting protein 19, inter-alpha-trypsin inhibi

Recovery

The recovery of rat ?-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-89
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ?-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	84-96
1:2	Average %	99
	Range %	92-105
1:4	Average %	104
	Range %	100-107
1:8	Average %	94
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.588	2.599	2.594	2.394
5	1.884	1.899	1.892	1.692
2.5	1.265	1.287	1.276	1.076
1.25	0.852	0.898	0.875	0.675
0.625	0.588	0.598	0.593	0.393
0.312	0.452	0.469	0.461	0.261
0.156	0.374	0.397	0.386	0.186
0	0.199	0.201	0.200	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q64240

Applications	ELISA
Research Area	AMBP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507561	CSB-EL001654RA	96T	$595	Add to Cart
BHE10507561	CSB-EL001654RA	5x96T	$2856	Add to Cart
BHE10507561	CSB-EL001654RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.