Matrices listed below were spiked with certain level of ALP and the recovery rates were calculated by comparing the measured value to the expected amount of ALP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
92
EDTA plasma(n=5)
88-100
93
heparin plasma(n=5)
91-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ALP ELISA Kit allows for the in vitro quantitative determination of ALP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ALP(Alkaline Phosphatase) ELISA Kit