Rat aldehyde dehydrogenase,ALDH ELISA Kit

Rat aldehyde dehydrogenase,ALDH ELISA Kit

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Mfr.No.

CSB-E11179r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

aldehyde dehydrogenase 2 family (mitochondrial), ALDH-E2, ALDHI, ALDM, MGC1806, ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH, mitochondrial aldehyde dehydrogenase 2, nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Recovery

The recovery of rat VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	86-99
EDTA plasma (n=4)	98	91-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	87
	Range %	81-92
1:400	Average %	104
	Range %	98-107
1:800	Average %	101
	Range %	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.084	0.088	0.086
100	0.152	0.159	0.156
50	0.226	0.234	0.230
25	0.389	0.378	0.384
12.5	0.613	0.635	0.624
6.25	0.830	0.843	0.837
3.12	1.138	1.117	1.128
0	1.970	1.869	1.920

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11884

Applications	ELISA
Research Area	ALDH2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10507555	CSB-E11179r	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.