Matrices listed below were spiked with certain level of AGE and the recovery rates were calculated by comparing the measured value to the expected amount of AGE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
94
EDTA plasma(n=5)
93-104
99
heparin plasma(n=5)
90-99
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AGE ELISA Kit allows for the in vitro quantitative determination of AGE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat AGE(Advanced glycation end-product) ELISA Kit