Rat AGE(Advanced Glycation End Product) ELISA Kit

Mfr.No.

ELK0785

Alternative Names

AGEs; Advanced Glycation End Products

Reactivity

Rat

Detection Range

125-8000 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

Recovery

Matrices listed below were spiked with certain level of recombinant AGE and the recovery rates were calculated by comparing the measured value to the expected amount of AGE in samples.

Matrix	Recovery range	Average
serum(n=5)	85-103%	94%
EDTA plasma(n=5)	95-105%	100%
Heparin plasma(n=5)	93-101%	97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	79-96%	89-101%	85-97%
EDTA plasma(n=5)	86-100%	93-108%	80-101%	87-96%
Heparin plasma(n=5)	86-93%	88-92%	86-97%	90-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
8000.00	0.235
4000.00	0.389
2000.00	0.672
1000.00	0.913
500.00	1.197
250.00	1.565
125.00	1.793
0.00	2.325

Sensitivity

38.2 ng/mL

Limit Of Detection

8000 ng/mL

Usage Notes	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208488	ELK0785	48T	$265		Add to Cart
BHE15208488	ELK0785	96T	$376		Add to Cart

Rat AGE(Advanced Glycation End Product) ELISA Kit

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