Rat advanced oxidation protein products(AOPP) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027429RA

Sensitivity

3.9 nmol/mL

Detection Range

15.6 nmol/mL-1000 nmol/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

advanced oxidation protein products(AOPP)

Recovery

The recovery of rat HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	96	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	84-96
1:2	Average %	95
	Range %	91-101
1:4	Average %	84
	Range %	81-89
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	AOPP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dexmedetomidine acts as an oxidative damage prophylactic in rats exposed to ionizing radiation Dilek Kutanis.et al,Journal of Clinical Anesthesia,34: 577-585. Antioxidant therapy improves non-thyroidal illness syndrome in uremic rats Yang P.et al,Ren Fail.,2016 May;38(4):514-20. Zhen-wu-tang ameliorates adenine-induced chronic renal failure in rats: regulation of the canonical Wnt4/beta-catenin signaling in the kidneys La L.et al,J Ethnopharmacol,Dec 14

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