Rat adrenocorticotropic hormone (ACTH) ELISA kit

Rat adrenocorticotropic hormone (ACTH) ELISA kit

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Mfr.No.

CSB-E06875r

Sensitivity

1.25 pg/mL

Detection Range

1.25 pg/mL-50 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

adrenocorticotropic hormone (ACTH)

Recovery

The recovery of mouse ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	99-104
EDTA plasma (n=4)	88	85-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	85
	Range %	81-89
1:200	Average %	92
	Range %	88-96
1:400	Average %	87
	Range %	82-92
1:800	Average %	100
	Range %	97-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.100	0.102	0.101
100	0.146	0.153	0.150
50	0.254	0.247	0.251
25	0.357	0.368	0.363
12.5	0.466	0.457	0.462
6.25	0.601	0.638	0.620
3.125	0.842	0.872	0.857
0	1.621	1.598	1.610

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ACTH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats Jin YB et al,J Radiat Res,2013 May 1;54(3):430-7 Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic-pituitary-adrenal axis in adult male rats Pereiro N et al,Toxicol Lett,2014 Jan 17.pii: S0378-4274(14)00012-5. Etomidate increases mortality inSeptic rats through inhibition of nuclear factor kappa-B rather than by causing adrenal insufficiency. Zhang Y.et al,J Surg Res.,2015 193(1):399-406. High-Cholesterol Diet Disrupts the Levels of Hormones Derived from Anterior Pituitary Basophilic Cells Yang J.et al,J Neuroendocrinol.,2016 ,28(3). Assessment of the role of intracranial hypertension and stress on hippocampal cell apoptosis and hypothalamic-pituitary dysfunction after TBI Tan H.et al,Sci Rep,Jun 19 A urinary metabolomics (GC-MS) strategy to evaluate the antidepressant-like effect of chlorogenic acid in adrenocorticotropic hormone-treated rats Le Zhao.et al,RSC Adv,Mar 1 Sini Decoction improves adrenal function and the short-term outcome of septic rats through downregulation of adrenal Toll-like receptor 4 expression Fang Lai.et al,/,/ Perfluorooctane sulfonate (PFOS) can alter the hypothalamic–pituitary–adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors R. Salgado.et al,Toxicology Letters,May 26 Transplantation of bone marrow mesenchymal stem cells reduces GluR1 expression in the hippocampus and amygdala and affects secretion of stress-related hormones in rats with spinal cord injuries: a potential anti-chronic stress mechanism Jia-Jia Sun.et al,INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,September 30 Exposure to cell phone radiofrequency changes corticotrophin hormone levels and histology of the brain and adrenal glands in male Wistar rat Sima Shahabi.et al,Iranian Journal of Basic Medical Sciences,July 16

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.