Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12

Mfr.No.

E1724Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12 (also known as ADAMTS12) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 1;ADAM-TS 1;ADAMTS-1;ADAM-TS1;ADAMTS12

Reactivity

Rat

Detection Range

0.5-100ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

105706

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.32ng/ml

Background

Cleaves aggrecan, a cartilage proteoglycan, at the '1683-Glu-|-Leu-1684' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).

Gene ID	79252
UniProt ID	Q9WUQ1
Antigen	ADAMTS12

Research Area

Signal Transduction; Cancer

Protocol

These standard curves of E1724Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	1.837	1.833	1.784
	1.83
32ng/ml	1.139	1.081	1.032
	1.023
16ng/ml	0.624	0.568	0.519
	0.511
8ng/ml	0.252	0.27	0.221
	0.288
4ng/ml	0.133	0.11	0.061
	0.087
0ng/ml	0.057	0.049	0
	0.041

Precision

We measured random samples of E1724Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.69	0.84	5.33
Intra-Assay	2	18	29.87	1.53	5.12
Intra-Assay	3	18	55.56	2.32	4.18

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12112930	E1724Ra	48T	$320		Add to Cart
BHE12112930	E1724Ra	96T	$458		Add to Cart

Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12 - ADAMTS12 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12 - ADAMTS12 ELISA Kit

Rate it now..!!