Rat adiponectin,ADP ELISA Kit

Rat adiponectin,ADP ELISA Kit

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Mfr.No.

CSB-E07271r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

adiponectin,ADP

Recovery

The recovery of goat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	90-103
1:4	Average %	91
	Range %	85-96
1:8	Average %	90
	Range %	88-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
60	0.149	0.144	0.147
15	0.289	0.298	0.294
4.8	0.520	0.527	0.524
1.2	0.861	0.865	0.863
0.3	1.411	1.416	1.414
0	2.213	2.212	2.213

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8K3R4

Applications	ELISA
Research Area	ADP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and production of pathogenic IgG-antibodies Dennis Wolf et al,PLOS ONE,2012 7(3):e33026. Comparison of the anti-obesity and hypocholesterolaemic effects of single Lactobacillus casei strain Shirota and probiotic cocktail Shafiq Aazmi et al,Food Science & Technology,2015 50(7):1589-1597 Fish oil supplementation alleviates depressant-like behaviors and modulates lipid profiles in rats exposed to chronic unpredictable mild stress Tang M.et al,BMC Complement Altern Med.,2015 ,15:239. Glycyrrhizic acid prevents high calorie diet-induced metabolic aberrations despite the suppression of peroxisome proliferator-activated receptor r expression Hong Sheng Cheng.et al,Nutrition,2016 online Anti-diabetic effects of soluble and insoluble dietary fibre from deoiled cumin in low-dose streptozotocin and high glucose-fat diet-induced type 2 diabetic rats Ma M.et al,Journal of Functional Foods,25: 186-196. Tetradecyl 2,3-Dihydroxybenzoate Improves the Symptoms of Diabetic Mice by Modulation of Insulin and Adiponectin Signaling Pathways Lan Xiang.et al,Front Pharmacol,Nov 13 Pepsin egg white hydrolysate ameliorates metabolic syndrome in high-fat/high-dextrose fed rats Silvia Moreno-Fernndez.et al,Food & Function,Oct 16 A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota Zhao L.et al,Food Funct.,Nov 20 Protective Effects of Ellagitannin-Rich Strawberry Extracts on Biochemical and Metabolic Disturbances in Rats Fed a Diet High in Fructose Bartosz Fotschki .et al,Nutrients,4 April

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.