Matrices listed below were spiked with certain level of ADAM10 and the recovery rates were calculated by comparing the measured value to the expected amount of ADAM10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
93
EDTA plasma(n=5)
87-105
100
heparin plasma(n=5)
90-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADAM10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-103%
96-101%
90-104%
EDTA plasma(n=5)
87-101%
89-101%
87-96%
heparin plasma(n=5)
88-100%
92-100%
87-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q10743
Applications
ELISA
Applications Notes
ADAM10 ELISA Kit allows for the in vitro quantitative determination of ADAM10 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ADAM10(Disintegrin and metalloproteinase domain-containing protein 10) ELISA Kit