Matrices listed below were spiked with certain level of AD and the recovery rates were calculated by comparing the measured value to the expected amount of AD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
85-104
95
heparin plasma(n=5)
91-101
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.