Rat activated coagulation factor X(FXa) ELISA Kit

Mfr.No.

CSB-EQ027251RA

Sensitivity

7.8 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

activated coagulation factor X(FXa)

Recovery

The recovery of rat ?-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	87	84-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ?-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	102
	Range %	97-107
1:4	Average %	101
	Range %	95-105
1:8	Average %	94
	Range %	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	FXa

Storage	Store at 2-8°C. Please refer to protocol.

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