Matrices listed below were spiked with certain level of ACTalpha2 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTalpha2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-104
98
EDTA plasma(n=5)
95-103
98
heparin plasma(n=5)
92-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTalpha2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ACTalpha2 ELISA Kit allows for the in vitro quantitative determination of ACTalpha2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ACTalpha2 (Actin Alpha 2, Smooth Muscle) ELISA Kit