Rat Acetyl-CoA Acetyltransferase - Cytosolic

Mfr.No.

E1569Ra

Sensitivity

3.36ng/L

Detection Range

6-1800ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Acetyl-CoA Acetyltransferase, Cytosolic (also known as ACAT2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Ab2-076;ACAT2;Acat3;Acetyl-CoA acetyltransferase, cytosolic;Cytosolic acetoacetyl-CoA thiolase

Molecular Weight

41108

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1569Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	524.44	14.21	2.71
Intra-Assay	2	18	290.99	12.39	4.26
Intra-Assay	3	18	190.68	10.45	5.48

Background

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT2 (acetyl-Coenzyme A acetyltransferase 2) gene Acetyl-Coenzyme A acetyltransferase 2 is an acetyl-CoA C-acetyltransferase enzyme.

Protocol

These standard curves of E1569Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.605	2.608	2.568
	2.611
480ng/L	1.509	1.501	1.461
	1.492
240ng/L	0.785	0.787	0.747
	0.788
120ng/L	0.426	0.413	0.374
	0.401
60ng/L	0.255	0.255	0.216
	0.256
0ng/L	0.045	0.04	0
	0.034

Gene ID	308100
UniProt ID	Q5XI22

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112796	E1569Ra	48T	$320		Add to Cart
BHE12112796	E1569Ra	96T	$458		Add to Cart

Rat Acetyl-CoA Acetyltransferase - Cytosolic - ACAT2 ELISA Kit

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