Matrices listed below were spiked with certain level of Acadm and the recovery rates were calculated by comparing the measured value to the expected amount of Acadm in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
92
EDTA plasma(n=5)
93-105
98
heparin plasma(n=5)
85-98
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acadm and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Acadm/Medium-chain specific acyl-CoA dehydrogenase, mitochondrial(MCAD)
Notes
For Research Use Only
UniProt ID
P08503
Antigen
ACADM
Applications
ELISA
Applications Notes
Acadm ELISA Kit allows for the in vitro quantitative determination of Acadm concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Acadm(Medium-chain specific acyl-CoA dehydrogenase, mitochondrial) ELISA Kit