Rat a1AT(Alpha-1-Antitrypsin) ELISA Kit

Mfr.No.

ELK6021

Alternative Names

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Reactivity

Rat

Detection Range

7.82-500 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant a1AT and the recovery rates were calculated by comparing the measured value to the expected amount of a1AT in samples.

Matrix	Recovery range	Average
serum(n=5)	84-95%	88%
EDTA plasma(n=5)	82-97%	92%
Heparin plasma(n=5)	87-99%	94%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of a1AT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-96%	79-95%	95-102%
EDTA plasma(n=5)	80-92%	86-102%	87-103%	85-101%
Heparin plasma(n=5)	87-96%	91-101%	87-98%	89-103%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
500.00	2.268	2.170
250.00	1.711	1.613
125.00	1.098	1.000
62.50	0.827	0.729
31.25	0.541	0.443
15.63	0.352	0.254
7.82	0.226	0.128
0.00	0.098	0.000

Sensitivity

3.5 ng/mL

Limit Of Detection

500 ng/mL

UniProt ID	P17475
Antigen	a1AT
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat a1AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat a1AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat a1AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat a1AT in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;Infection immunity;Hepatology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205125	ELK6021	48T	$320		Add to Cart
BHE15205125	ELK6021	96T	$458		Add to Cart

Rat a1AT(Alpha-1-Antitrypsin) ELISA Kit

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