Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit

Mfr.No.

ER0199

Alternative Names

5-LO ELISA Kit, Alox5 ELISA Kit, 5-LO ELISA Kit, 5LPG ELISA Kit, LOG5 ELISA Kit, LOX5A ELISA Kit, 5-lipoxygenase ELISA Kit, 5-LOX ELISA Kit, arachidonic 5-lipoxygenase alpha-10 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-10-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-p10 isoform ELISA Kit, arachidonic acid 5-lipoxygenase ELISA Kit, leukotriene A4 synthase ELISA Kit, LOG5 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of 5-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 5-LO in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-102	94
EDTA plasma(n=5)	92-100	97
heparin plasma(n=5)	86-105	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-102%	86-105%	86-99%
EDTA plasma(n=5)	83-99%	89-100%	82-95%
heparin plasma(n=5)	84-95%	80-100%	80-95%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

5-LO/Alox5/5-LO/5LPG/LOG5/LOX5A/5-lipoxygenase/5-LOX/arachidonic 5-lipoxygenase alpha-10 isoform/arachidonic 5-lipoxygenase delta-10-13 isoform/arachidonic 5-lipoxygenase delta-13 isoform/arachidonic 5-lipoxygenase delta-p10 isoform/arachidonic acid 5-lipoxygenase/leukotriene A4 synthase/LOG5

Notes

For Research Use Only

UniProt ID	P12527
Antigen	5-LO

Applications	ELISA
Applications Notes	5-LO ELISA Kit allows for the in vitro quantitative determination of 5-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Evidence-Based Complementary and Alternative Medicine Title: Tualang Honey Reduced Neuroinflammation and Caspase-3 Activity in Rat Brain after Kainic Acid-Induced Status Epilepticus

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806060	ER0199	96T	$400		Add to Cart

Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit

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