Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13983r

Sensitivity

25 ng/mL

Detection Range

25 ng/mL-400 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

5-hydroxyindolacetic acid,5-HIAA

Recovery

The recovery of human FLOT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-100
EDTA plasma (n=4)	95	90-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FLOT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	98
	Range %	92-102
1:4	Average %	95
	Range %	88-100
1:8	Average %	94
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	5-HIAA

Storage	Store at 2-8°C. Please refer to protocol.

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