Rat 4-Hydroxynonenal(HNE)ELISA Kit

Mfr.No.

CSB-EQ027232RA

Alternative Names

4-Hydroxynonenal(HNE)

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human CYP2C9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	81-94
EDTA plasma (n=4)	105	97-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2C9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	105
	Range %	94-109
1:2	Average %	97
	Range %	89-101
1:4	Average %	93
	Range %	83-98
1:8	Average %	107
	Range %	94-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.497	2.389	2.443	2.277
750	1.795	1.767	1.781	1.615
375	1.076	1.062	1.069	0.903
187.5	0.605	0.595	0.600	0.434
94	0.376	0.366	0.371	0.205
47	0.248	0.258	0.253	0.087
23.5	0.215	0.211	0.213	0.047
0	0.169	0.163	0.166	?

Species

Rattus norvegicus

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

Antigen	4-Hydroxynonenal(HNE)

Applications

ELISA

Research Area

HNE

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Age-dependent changes of the antioxidant system in rat liver are accompanied by altered MAPK activation and aDecline in mTOR signaling Wei Yang. et al,EXCLI Journal ,2015 14:1273-1290 Titanium Dioxide Nanoparticles Provoke Testicular Injury and Apoptosis in Sperm through Oxidative Stress-Activated Mitochondrial and Death Receptor Pathways Mohamed A El-Missiry.et al,Imperial Journal of Interdisciplinary Research ,Nov 11 Bone turnover and oxidative stress markers in estrogendeficient rats treated with Marantodes pumilum leaves and roots: A comparative study Tijjani Rabiu Giaze.et al,TROP J PHARM RES,/

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10507499	CSB-EQ027232RA	96T	$790	Add to Cart
BHE10507499	CSB-EQ027232RA	5x96T	$3792	Add to Cart
BHE10507499	CSB-EQ027232RA	10x96T	$7505	Add to Cart

Rat 4-Hydroxynonenal(HNE)ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat 4-Hydroxynonenal(HNE)ELISA Kit

Rate it now..!!