Rat 3-Hydroxy-3-Methylglutaryl-coenzyme A Synthase

Mfr.No.

E1834Ra

Sensitivity

0.048ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

3H3M;3-hydroxy-3-methylglutaryl-coenzyme A reductase;HMG-CoA reductase;HMGCS

Molecular Weight

96688

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1834Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.8	0.58	4.51
Intra-Assay	2	18	18.97	0.53	2.81
Intra-Assay	3	18	4.75	0.22	4.54

Background

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis.

Protocol

These standard curves of E1834Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	1.906	2.024	1.985
	2.143
12ng/mL	0.937	0.97	0.93
	1.003
6ng/mL	0.551	0.516	0.477
	0.481
3ng/mL	0.209	0.209	0.17
	0.209
1.5ng/mL	0.156	0.123	0.084
	0.09
0ng/mL	0.043	0.039	0
	0.035

Gene ID	25675
UniProt ID	P51639

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113033	E1834Ra	48T	$320		Add to Cart
BHE12113033	E1834Ra	96T	$458		Add to Cart

Rat 3-Hydroxy-3-Methylglutaryl-coenzyme A Synthase - HMGCS ELISA Kit

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