Rat 17-beta-Hydroxysteroid Dehydrogenase 13

Mfr.No.

E1832Ra

Sensitivity

0.019ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

17-beta-HSD 13;17-beta-hydroxysteroid dehydrogenase 13;HSD17B13;Short-chain dehydrogenase/reductase 9

Molecular Weight

33494

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1832Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.11	0.09	7.86
Intra-Assay	2	18	2.58	0.17	6.46
Intra-Assay	3	18	6.41	0.43	6.66

Background

17beta-Hydroxysteroid dehydrogenase type 13 also known as 17beta-HSD type 13 is an enzyme that in humans is encoded by the HSD17B13 gene. 17beta-HSD13 is significantly up-regulated in the liver of patients with non-alcoholic fatty liver disease (NAFLD) and enhances lipogenesis.

Protocol

These standard curves of E1832Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.329	2.32	2.279
	2.31
6ng/mL	1.608	1.656	1.616
	1.704
3ng/mL	1.049	1.107	1.066
	1.165
1.5ng/mL	0.765	0.733	0.692
	0.701
0.75ng/mL	0.485	0.455	0.414
	0.424
0ng/mL	0.051	0.041	0
	0.03

Gene ID	305150
UniProt ID	Q5M875

Research Area	Signal Transduction; Cell Biology; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113031	E1832Ra	48T	$320		Add to Cart
BHE12113031	E1832Ra	96T	$458		Add to Cart

Rat 17-beta-Hydroxysteroid Dehydrogenase 13 - HSD17B13 ELISA Kit

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