Rat 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Gamma-1

Mfr.No.

E1833Ra

Sensitivity

7.26ng/L

Detection Range

15-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-gamma-1;PLCG1;PLC-gamma-1;PPLCA

Molecular Weight

148548

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1833Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	568.66	24.28	4.27
Intra-Assay	2	18	229.43	17.2	7.5
Intra-Assay	3	18	189.55	15.82	8.34

Background

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:7531435). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:7531435). Plays a role in actin reorganization and cell migration (By similarity).

Protocol

These standard curves of E1833Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.357	2.201	2.162
	2.044
800ng/L	1.541	1.568	1.529
	1.595
400ng/L	1.039	1.004	0.965
	0.969
200ng/L	0.721	0.725	0.687
	0.729
100ng/L	0.441	0.467	0.429
	0.494
0ng/L	0.028	0.039	0
	0.05

Gene ID	25738
UniProt ID	P10686

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113032	E1833Ra	48T	$320		Add to Cart
BHE12113032	E1833Ra	96T	$458		Add to Cart

Rat 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Gamma-1 - PLCG1 ELISA Kit

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