Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Mfr.No.

CSB-E08524Rb

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Vascular Endothelial cell Growth Factor,VEGF

Recovery

The recovery of human PSG9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-100
EDTA plasma (n=4)	96	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PSG9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	89
	Range %	83-99
1:4	Average %	98
	Range %	90-105
1:8	Average %	92
	Range %	88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	VEGF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Intravitreal bevacizumab effects on VEGF levels in distant organs: an experimental study Dinc E et al,Cutan Ocul Toxicol,2013 Nov 14. Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model S Huang.et al,Experimental and Therapeutic Medicine,2015 177-182 Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015 Sep;20(9):1164-75 The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoagulation in pigmented rabbits Arimura S.et al,Experimental Eye Research,149,1-7 Photocoagulation of the Retinal Nonperfusion Area Prevents the Expression of the Vascular Endothelial Growth Factor in an Animal Model Gozawa M.et al,Invest Ophthalmol Vis Sci,Nov 1 Construction of engineered corpus caveosum with primary mesenchymal stem cells in vitro Xie X.et al,Sci Rep,Dec 22 Panretinal photocoagulation using short-pulse laser induces less inflammation and macular thickening in patients with diabetic retinopathy Yoshihiro Takamura.et al,University of Fukui,/ Effect of 0.05% Difluprednate Ophthalmic Emulsion on Proinflammatory Cytokine Levels After Retinal Laser Photocoagulation in Rabbits Kakimoto Hiroshi.et al,Joual of Ocular Pharmacology and Therapeutics,May 29

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