Rabbit Tumor necrosis factor alpha,TNFalpha ELISA Kit

Rabbit Tumor necrosis factor alpha,TNFalpha ELISA Kit

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Mfr.No.

CSB-E06998Rb

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (TNF superfamily, member 2), DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of human P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	96	87-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	86
	Range %	80-92
1:10	Average %	97
	Range %	91-105
1:20	Average %	98
	Range %	92-110
1:40	Average %	95
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.305	2.368	2.337	2.269
30	1.795	1.805	1.800	1.732
15	1.146	1.187	1.167	1.099
7.5	0.653	0.689	0.671	0.603
3.75	0.344	0.358	0.351	0.283
1.8	0.245	0.264	0.255	0.187
0.9	0.110	0.116	0.113	0.045
0	0.066	0.069	0.068	?

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04924

Applications	ELISA
Research Area	TNF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507477	CSB-E06998Rb	96T	$615	Add to Cart
BHE10507477	CSB-E06998Rb	5x96T	$2952	Add to Cart
BHE10507477	CSB-E06998Rb	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.