Matrices listed below were spiked with certain level of TNF-beta and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-beta in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
94
EDTA plasma(n=5)
96-101
98
heparin plasma(n=5)
88-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
88-104%
85-103%
EDTA plasma(n=5)
82-98%
83-98%
84-100%
heparin plasma(n=5)
88-98%
81-97%
83-96%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P10154
Applications
ELISA
Applications Notes
TNF-beta ELISA Kit allows for the in vitro quantitative determination of TNF-beta concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rabbit TNF-beta(Tumor Necrosis Factor beta) ELISA Kit