Matrices listed below were spiked with certain level of TGF-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of TGF-alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
100
EDTA plasma(n=5)
92-100
98
heparin plasma(n=5)
86-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGF-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-101%
90-103%
86-104%
EDTA plasma(n=5)
82-99%
88-96%
84-96%
heparin plasma(n=5)
82-99%
85-91%
93-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P98138
Applications
ELISA
Applications Notes
TGF-alpha ELISA Kit allows for the in vitro quantitative determination of TGF-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rabbit TGF-alpha(Transforming Growth Factor Alpha) ELISA Kit