Rabbit terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Mfr.No.

CSB-E15838Rb

Sensitivity

1.95 ng/mL

Detection Range

7.8 ng/mL-500 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

terminal complement complex C5b-9 (TCC C5b-9)

Recovery

The recovery of mouse amylin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse amylin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	107
	Range %	103-110
1:2	Average %	89
	Range %	85-93
1:4	Average %	102
	Range %	98-106
1:8	Average %	85
	Range %	81-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	TCC C5b-9

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Differential Interactive Effects of Cartilage Traumatization and Blood Exposure In Vitro and In Vivo Joos H. et al,Am J Sports Med,2015 Sep 11.pii: 0363546515602248.

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