Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15043Rb

Sensitivity

19.5 uU/mL

Detection Range

78 uU/mL-5000 uU/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Tartrate-resistant acid phosphatase 5b (TRAP5b)

Recovery

The recovery of human Ang-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	98
	Range %	94-103
1:4	Average %	105
	Range %	100-110
1:8	Average %	108
	Range %	102-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O97860

Applications	ELISA
Research Area	TRAP5b

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tetrandrine attenuates the bone erosion in collagen-induced arthritis rats by inhibiting osteoclastogenesis via spleen tyrosine kinase Yugai Jia.et al,The FASEB Journal,Jan 30

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