Matrices listed below were spiked with certain level of 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-103
98
EDTA plasma(n=5)
89-104
97
heparin plasma(n=5)
91-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-101%
88-105%
87-104%
EDTA plasma(n=5)
90-101%
90-101%
93-101%
heparin plasma(n=5)
95-100%
94-100%
94-97%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ST/5-HT ELISA Kit allows for the in vitro quantitative determination of 5-HT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rabbit ST/5-HT(5-hydroxytryptamine) ELISA Kit