Rabbit soluble P-selectin,sP-selectin ELISA Kit

Rabbit soluble P-selectin,sP-selectin ELISA Kit

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Mfr.No.

CSB-E06861Rb

Alternative Names

soluble P-selectin,sP-selectin

Reactivity

Rabbit

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Urine, Cell culture supernatants, Saliva

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human RBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-98
1:200	Average %	98
	Range %	93-104
1:400	Average %	84
	Range %	82-88
1:800	Average %	90
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.634	2.702	2.668	2.566
500	2.234	2.217	2.226	2.124
250	1.496	1.527	1.512	1.410
125	0.856	0.879	0.868	0.766
62.5	0.446	0.468	0.457	0.355
31.2	0.267	0.285	0.276	0.174
15.6	0.206	0.212	0.209	0.107
0	0.105	0.099	0.102	?

Species

Oryctolagus cuniculus

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

Antigen	soluble P-selectin,sP-Selectin

Applications

ELISA

Research Area

sP-selectin

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dyslipidemia induced inflammatory status, platelet activation and endothelial dysfunction in rabbits: Protective role of 10-Dehydrogingerdione Mohamed MahmoudEl-Seweidy, et al,Biomedicine & Pharmacotherapy,December 5

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BHE10507457	CSB-E06861Rb	10x96T	$6251	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.