Rabbit SFRP5 ELISA Kit PicoKine®

Mfr.No.

EK1472-RB

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rabbit

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.056	0.168	0.294	0.516	0.953	1.487	1.912	2.189

Description

Rabbit SFRP5 ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit SFRP5 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Secreted frizzled-related protein 5;sFRP-5;Frizzled-related protein 1b;FRP-1b;Secreted apoptosis-related protein 3;SARP-3;SFRP5;FRP1B, SARP3;

Molecular Weight

35563 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: E30-H317

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	749	2783	6025	877	2948	7356
Standard deviation	53.93	178.11	439.82	74.54	256.47	698.82
CV (%)	7.2	6.4	7.3	8.5	8.7	9.5

Protein Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Background

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100355147
Gene Name	SFRP5
UniProt ID	Q5T4F7
Protein Name	Secreted frizzled-related protein 5
Tissue Specificity	Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle. .

Applications	ELISA
Research Area	Secreted,Signaling Pathways,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001228	EK1472-RB	96 wells/kit	$750		Add to Cart

Rabbit SFRP5 ELISA Kit PicoKine®

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