Rabbit RBP5(Retinol Binding Protein 5, Cellular) ELISA Kit

Mfr.No.

ELK7747

Alternative Names

CRBPIII; HRBPiso; Cellular retinol-binding protein III

Reactivity

Rabbit

Detection Range

1.57-100 ng/mL

Sample Type

Cell lysates, Serum, Cell culture supernatants, Plasma, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant RBP5 and the recovery rates were calculated by comparing the measured value to the expected amount of RBP5 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-95%	86%
EDTA plasma(n=5)	80-96%	88%
Heparin plasma(n=5)	80-92%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RBP5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-99%	88-104%	84-98%	95-105%
EDTA plasma(n=5)	95-104%	87-96%	92-101%	82-90%
Heparin plasma(n=5)	78-92%	83-96%	87-98%	95-102%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	2.052	1.969
50.00	1.507	1.424
25.00	1.240	1.157
12.50	0.839	0.756
6.25	0.573	0.490
3.13	0.363	0.280
1.57	0.193	0.110
0.00	0.083	0.000

Sensitivity

0.67 ng/mL

Limit Of Detection

100 ng/mL

Antigen	RBP5
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit RBP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit RBP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit RBP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit RBP5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Signal transduction;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207267	ELK7747	48T	$412		Add to Cart
BHE15207267	ELK7747	96T	$588		Add to Cart

Rabbit RBP5(Retinol Binding Protein 5, Cellular) ELISA Kit

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