Rabbit Pulmonary Surfatcant-associated Protein B

Mfr.No.

E0075Rb

Sensitivity

0.27ng/mL

Detection Range

0.5-200ng/mL

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

6 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTPB;SP-B

Molecular Weight

40610

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0075Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	47.9	1.84	3.8
Intra-Assay	2	18	8.8	0.59	6.7
Intra-Assay	3	18	23.8	1.61	6.8

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Protocol

These standard curves of E0075Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/mL	2.294	2.212	2.167
	2.129
60ng/mL	1.601	1.595	1.55
	1.588
30ng/mL	0.982	1.061	1.017
	1.14
15ng/mL	0.639	0.688	0.644
	0.738
7.5ng/mL	0.49	0.446	0.402
	0.403
0ng/mL	0.029	0.044	0
	0.06

Gene ID	100009194
UniProt ID	P15285

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111061	E0075Rb	48T	$320		Add to Cart
BHE12111061	E0075Rb	96T	$458		Add to Cart

Rabbit Pulmonary Surfatcant-associated Protein B - SP-B ELISA Kit

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