Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

Mfr.No.

CSB-EL021173RB

Alternative Names

surfactant protein B, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, OTTHUMP00000203253, Pulmonary surfactant-associated protein B, 18kD, surfactant, pulmonary-associated protein B

Reactivity

Rabbit

Detection Range

1.87 ng/mL-120 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	88	84-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	102
	Range %	98-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.890	1.952	1.921	1.766
500	1.338	1.316	1.327	1.172
250	0.924	0.904	0.914	0.759
125	0.585	0.599	0.592	0.437
62.5	0.402	0.424	0.413	0.258
31.25	0.321	0.309	0.315	0.160
15.6	0.238	0.229	0.234	0.079
0	0.153	0.157	0.155	?

Species

Oryctolagus cuniculus

Sensitivity

0.46 ng/mL

Limit Of Detection

450 nm

UniProt ID	P15285
Antigen	surfactant protein B

Applications

ELISA

Research Area

SFTPB

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507449	CSB-EL021173RB	96T	$790	Add to Cart
BHE10507449	CSB-EL021173RB	5x96T	$3792	Add to Cart
BHE10507449	CSB-EL021173RB	10x96T	$7505	Add to Cart

Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

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