Matrices listed below were spiked with certain level of PCSK9 and the recovery rates were calculated by comparing the measured value to the expected amount of PCSK9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
97
EDTA plasma(n=5)
88-101
97
heparin plasma(n=5)
96-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCSK9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PCSK9 ELISA Kit allows for the in vitro quantitative determination of PCSK9 concentrations in serum, plasma, tissue homogenates and other biological fluids.