Matrices listed below were spiked with certain level of N-MID-OT and the recovery rates were calculated by comparing the measured value to the expected amount of N-MID-OT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
94
EDTA plasma(n=5)
88-100
93
heparin plasma(n=5)
89-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-MID-OT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
85-94%
87-102%
EDTA plasma(n=5)
83-96%
84-101%
84-100%
heparin plasma(n=5)
83-99%
84-99%
82-95%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
P39056
Applications
ELISA
Applications Notes
N-MID-OT ELISA Kit allows for the in vitro quantitative determination of N-MID-OT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rabbit N-MID-OT (N-MID Osteocalcin) ELISA Kit