Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

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Mfr.No.

CSB-E06921Rb

Alternative Names

chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Reactivity

Rabbit

Detection Range

23.5 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-99
EDTA plasma (n=4)	96	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	98
	Range %	93-105
1:4	Average %	88
	Range %	85-92
1:8	Average %	90
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.823	2.988	2.906	2.747
15	2.120	2.230	2.175	2.016
7.5	1.509	1.504	1.507	1.348
3.75	0.842	0.891	0.867	0.708
1.8	0.518	0.555	0.537	0.378
0.9	0.386	0.382	0.384	0.225
0.45	0.260	0.254	0.257	0.098
0	0.157	0.160	0.159

Species

Oryctolagus cuniculus

Sensitivity

5.87 pg/mL

Limit Of Detection

450 nm

UniProt ID	P28292
Antigen	chemokine (C-C motif) ligand 2

Applications

ELISA

Research Area

CCL2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507413	CSB-E06921Rb	96T	$615	Add to Cart
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BHE10507413	CSB-E06921Rb	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.