Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

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Mfr.No.

CSB-E09269Rb

Alternative Names

matrix metallopeptidase 3 (stromelysin 1, progelatinase), CHDS6, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1, matrix metalloproteinase 3, matrix metalloproteinase 3 (stromelysin 1, progelatinase), proteoglycanase, transin-1

Reactivity

Rabbit

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	84-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	93
	Range %	89-97
1:8	Average %	90
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
2000	2.362	2.458	2.410	2.229
1000	1.943	1.831	1.887	1.706
500	1.206	1.241	1.224	1.043
250	0.754	0.767	0.761	0.580
125	0.395	0.413	0.404	0.223
62.5	0.318	0.335	0.327	0.146
31.25	0.221	0.234	0.228	0.047
0	0.179	0.182	0.181

Species

Oryctolagus cuniculus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P28863
Antigen	matrix metallopeptidase 3 (stromelysin 1, progelatinase)

Applications

ELISA

Research Area

MMP3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507409	CSB-E09269Rb	96T	$445	Add to Cart
BHE10507409	CSB-E09269Rb	5x96T	$2136	Add to Cart
BHE10507409	CSB-E09269Rb	10x96T	$4227.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.