Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

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Mfr.No.

CSB-E10373Rb

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), CLG4, CLG4A, MMP-II, MONA, TBE-1, collagenase type IV-A, matrix metalloproteinase 2, matrix metalloproteinase-II, neutrophil gelatinase

Recovery

The recovery of rat MMP8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	90
	Range %	85-94
1:4	Average %	88
	Range %	84-92
1:8	Average %	102
	Range %	98-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.620	2.459	2.540	2.363
10	2.056	2.146	2.101	1.924
5	1.383	1.432	1.408	1.231
2.5	0.825	0.801	0.813	0.636
1.25	0.509	0.518	0.514	0.337
0.625	0.417	0.405	0.411	0.234
0.312	0.251	0.257	0.254	0.077
0	0.180	0.173	0.177

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50757

Applications	ELISA
Research Area	MMP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507408	CSB-E10373Rb	96T	$495	Add to Cart
BHE10507408	CSB-E10373Rb	5x96T	$2376	Add to Cart
BHE10507408	CSB-E10373Rb	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.