Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Mfr.No.

CSB-E14220Rb

Alternative Names

matrix metallopeptidase 13 (collagenase 3), CLG3, MANDP1, collagenase 3, matrix metalloproteinase 13, matrix metalloproteinase 13 (collagenase 3)

Reactivity

Rabbit

Detection Range

93.75 pg/mL-6000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-107
EDTA plasma (n=4)	106	98-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-97
1:2	Average %	98
	Range %	91-101
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Sensitivity

23.44 pg/mL

Limit Of Detection

450 nm

UniProt ID	O62806
Antigen	matrix metallopeptidase 13 (collagenase 3)

Applications

ELISA

Research Area

MMP13

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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