Rabbit Interleukin 8 - IL-8 ELISA Kit

Mfr.No.

E0044Rb

Sensitivity

0.57ng/L

Detection Range

1-400ng/L

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 8 (also known as IL-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;IL 8;IL8;IL-8;Interleukin-8;NAP-1;Neutrophil attractant/activation protein 1;Permeability factor 1;PF1;rPF1

Molecular Weight

11403

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0044Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	77.5	6.1	7.9
Intra-Assay	2	18	116.9	5.06	4.3
Intra-Assay	3	18	17.3	1.15	6.6

Background

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Protocol

These standard curves of E0044Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.038	2.168	2.114
	2.298
120ng/L	1.477	1.56	1.505
	1.643
60ng/L	1.108	1.062	1.007
	1.015
30ng/L	0.657	0.687	0.632
	0.717
15ng/L	0.46	0.453	0.398
	0.447
0ng/L	0.058	0.055	0
	0.052

Gene ID	100009129
UniProt ID	P19874

Research Area	Cardiovascular; Immunology; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111031	E0044Rb	48T	$320		Add to Cart
BHE12111031	E0044Rb	96T	$458		Add to Cart

Rabbit Interleukin 8 - IL-8 ELISA Kit

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