Rabbit Interleukin 1beta,IL-1beta ELISA Kit

Mfr.No.

CSB-E06900Rb

Alternative Names

interleukin 1, beta, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta

Reactivity

Rabbit

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PXDN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PXDN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-105
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	P14628
Antigen	interleukin 1, beta

Applications

ELISA

Research Area

IL1B

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507388	CSB-E06900Rb	96T	$658	Add to Cart
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BHE10507388	CSB-E06900Rb	10x96T	$6251	Add to Cart